LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1565..1661) /label=attL2 /note="attL2 (c)" misc_signal complement(1676..1695) /note="T7 Promoter/priming site (c)" primer_bind 1703..1719 /note="M13 Reverse priming site" gene 1832..2641 /note="Kanamycin resistance gene" rep_origin 2762..3435 /note="pUC origin" vector join(1573..3438,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..1558 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:9183 IMAGE:3925036" /tissue_type="Skin, melanotic melanoma." /clone_lib="NIH_MGC_72" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 692..1558 /gene="ST3GAL1" /gene_synonym=Gal-NAc6S /gene_synonym=MGC9183 /gene_synonym=SIATFL /gene_synonym=ST3GalA.1 /gene_synonym=ST3GalIA /gene_synonym=ST3O /db_xref="GeneID:6482" /db_xref="HGNC:10862" /db_xref="MIM:607187" CDS 692..1558 /dnas_title="ST3 beta-galactoside alpha-2,3-sialyltransferase 1" /gene="ST3GAL1" /gene_synonym=Gal-NAc6S /gene_synonym=MGC9183 /gene_synonym=SIATFL /gene_synonym=ST3GalA.1 /gene_synonym=ST3GalIA /gene_synonym=ST3O /codon_start=1 /product="ST3 beta-galactoside alpha-2,3-sialyltransferase 1" /protein_id="AAH18357.1" /db_xref="GI:22450876" /db_xref="GeneID:6482" /db_xref="HGNC:10862" /db_xref="MIM:607187" /translation="MVTLRKRTLKVLTFLVLFIFLTSFFLNYSHTMVATTWFPKQMVL ELSENLKRLIKHRPCTCTHCIGQRKLSAWFDERFNQTMQPLLTAQNALLEDDTYRWWL RLQREKKPNNLNDTIKELFRVVPGNVDPMLEKRSVGCRRCAVVGNSGNLRESSYGPEI DSHDFVLRMNKAPTAGFEADVGTKTTHHLVYPESFRELGDNVSMILVPFKTIDLEWVV SAITTGTISHTYIPVPAKIRVKQDKILIYHPAFIKYVFDNWLQGHGRYPSTGILSVIF SMHVCDEVDLYGFGADSKGNWHHYWENNPSAGAFRKTGVHDADFESNVTATLASINKI RIFKGR" misc_difference 799..799 /gene="ST3GAL1" /gene_synonym=Gal-NAc6S /gene_synonym=MGC9183 /gene_synonym=SIATFL /gene_synonym=ST3GalA.1 /gene_synonym=ST3GalIA /gene_synonym=ST3O /note="'T' in cDNA is 'C' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cagactgatc aagcacaggc cttgcacctg 721 cacccactgc atcgggcagc gcaagctctc ggcctggttc gatgagaggt tcaaccagac 781 catgcagccg ctgctgactg cccagaacgc gctcttggag gacgacacct accgatggtg 841 gctgaggctc cagcgggaga agaagcccaa taacttgaat gacaccatca aggagctgtt 901 cagagtggtg cctgggaatg tggaccctat gctggagaag aggtcggtgg gctgccggcg 961 ctgcgccgtt gtgggcaact cgggcaacct gagggagtct tcttatgggc ctgagataga 1021 cagtcacgac tttgtcctca ggatgaacaa ggcgcccacg gcagggtttg aagctgatgt 1081 tgggaccaag accacccacc atctggtgta ccctgagagc ttccgggagc tgggagataa 1141 tgtcagcatg atcctggtgc ccttcaagac catcgacttg gagtgggtgg tgagcgccat 1201 caccacgggc accatttccc acacctacat cccggttcct gcaaagatca gagtgaaaca 1261 ggataagatc ctgatctacc acccagcctt catcaagtat gtctttgaca actggctgca 1321 agggcacggg cgatacccat ctaccggcat cctctcggtc atcttctcaa tgcatgtctg 1381 cgatgaggtg gacttgtacg gcttcggggc agacagcaaa gggaactggc accactactg 1441 ggagaacaac ccatccgcgg gggcttttcg caagacgggg gtgcacgatg cagactttga 1501 gtctaacgtg acggccacct tggcctccat caataaaatc cggatcttca aggggagaTA 1561 GGACCCAGCT TTCTTGTACA AAGTTGGCAT TATAAGAAAG CATTGCTTAT CAATTTGTTG 1621 CAACGAACAG GTCACTATCA GTCAAAATAA AATCATTATT TGCCATCCAG CTGATATCCC 1681 CTATAGTGAG TCGTATTACA TGGTCATAGC TGTTTCCTGG CAGCTCTGGC CCGTGTCTCA 1741 AAATCTCTGA TGTTACATTG CACAAGATAA AATAATATCA TCATGAACAA TAAAACTGTC 1801 TGCTTACATA AACAGTAATA CAAGGGGTGT TATGAGCCAT ATTCAACGGG AAACGTCGAG 1861 GCCGCGATTA AATTCCAACA TGGATGCTGA TTTATATGGG TATAAATGGG CTCGCGATAA 1921 TGTCGGGCAA TCAGGTGCGA CAATCTATCG CTTGTATGGG AAGCCCGATG CGCCAGAGTT 1981 GTTTCTGAAA CATGGCAAAG GTAGCGTTGC CAATGATGTT ACAGATGAGA TGGTCAGACT 2041 AAACTGGCTG ACGGAATTTA TGCCTCTTCC GACCATCAAG CATTTTATCC GTACTCCTGA 2101 TGATGCATGG TTACTCACCA CTGCGATCCC CGGAAAAACA GCATTCCAGG TATTAGAAGA 2161 ATATCCTGAT TCAGGTGAAA ATATTGTTGA TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA 2221 TTCGATTCCT GTTTGTAATT GTCCTTTTAA CAGCGATCGC GTATTTCGTC TCGCTCAGGC 2281 GCAATCACGA ATGAATAACG GTTTGGTTGA TGCGAGTGAT TTTGATGACG AGCGTAATGG 2341 CTGGCCTGTT GAACAAGTCT GGAAAGAAAT GCATAAACTT TTGCCATTCT CACCGGATTC 2401 AGTCGTCACT CATGGTGATT TCTCACTTGA TAACCTTATT TTTGACGAGG GGAAATTAAT 2461 AGGTTGTATT GATGTTGGAC GAGTCGGAAT CGCAGACCGA TACCAGGATC TTGCCATCCT 2521 ATGGAACTGC CTCGGTGAGT TTTCTCCTTC ATTACAGAAA CGGCTTTTTC AAAAATATGG 2581 TATTGATAAT CCTGATATGA ATAAATTGCA GTTTCATTTG ATGCTCGATG AGTTTTTCTA 2641 ATCAGAATTG GTTAATTGGT TGTAACACTG GCAGAGCATT ACGCTGACTT GACGGGACGG 2701 CGCAAGCTCA TGACCAAAAT CCCTTAACGT GAGTTACGCG TCGTTCCACT GAGCGTCAGA 2761 CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG 2821 CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC 2881 AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT 2941 AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC 3001 TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT 3061 GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG 3121 CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT 3181 ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG 3241 GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG 3301 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG 3361 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG 3421 GCCTTTTGCT CACATGTT //